Gene for staphylococcal protein A.
نویسندگان
چکیده
منابع مشابه
Complete sequence of the staphylococcal gene encoding protein A. A gene evolved through multiple duplications.
The gene coding for protein A from Staphylococcus aureus has been isolated by molecular cloning, and a subclone containing an 1.8-kilobase insert was found to give a functional protein A in Escherichia coli. The complete nucleotide sequence of the insert, including the structural gene and the 5' and 3' flanking sequences, has been determined. Starting from a TTG initiator codon, an open reading...
متن کاملAnalysis of signals for secretion in the staphylococcal protein A gene.
Different constructs of the gene encoding staphylococcal protein A were introduced in Staphylococcus aureus and S. xylosus as well as Escherichia coli. The product of the gene without the cell wall anchoring domain was efficiently secreted in all three hosts. N-terminal sequencing of the affinity-purified mature protein revealed a common processing site after the alanine residue at position 36....
متن کاملStaphylococcal Protein A as a Pharmacological Treatment for Autoimmune Disorders
Staphylococcal protein A (SpA) is a key virulence factor that enables Staphylococcus aureus to evade host innate and adaptive immune responses. The immunomodulatory properties of SpA have led to a hypothesis that it may have pharmacological applications as a treatment for autoimmune disease. Clinical trials are underway to test whether ultrapure SpA can be used to treat immune thrombocytopenia ...
متن کاملEffect of protein A on staphylococcal opsonization.
To study the effect of wall protein A on bacterial opsonization, phagocytosis of 10 strains of Staphylococcus aureus with high and low protein A contents was measured. Those strains that contained the highest concentrations of protein A were phagocytized by human neutrophils at a slower rate than strains with little or no protein A when normal human serum and purified immunoglobulin G (IgG) wer...
متن کاملA highly sensitive assay for proteases using staphylococcal protein A fused with enhanced green fluorescent protein.
Enhanced green fluorescent protein (EGFP) was fused with staphylococcal protein A (SpA) and used as a substrate for proteases. An SpA-EGFP assay was done in three steps: (i) digestion of SpA-EGFP by proteases, (ii) addition of rabbit IgG immobilized on Sepharose beads, and (iii) measurement of the fluorescence intensity of supernatant. The assay was sensitive enough to measure picogram levels o...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1983
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.80.3.697